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Induction of micronuclei and nuclear abnormalities by cyclophosphamide and colchicine in Xenotoca melanosoma (Pisces, Goodeidae) from Lake La Alberca in Michoacan, Mexico.

Research paper by Jose Luis JL Zavala-Aguirre, Olivia O Torres-Bugarin, Hector Rene HR Buelna-Osben, Lola Paulina LP Flores-Kehn, Maria Luisa ML Ramos-Ibarra, Guillermo G Zuniga-Gonzalez, Tetsuya T Ogura

Indexed on: 15 Apr '10Published on: 15 Apr '10Published in: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering



Abstract

This study is a follow-up of previous research in which we described the frequency of spontaneous micronucleated erythrocytes (MNE) in the Goodeid Xenotocoa melanosoma collected from Lake La Alberca, located in the state of Michoacan, Mexico. In the present work, we measured micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of peripheral blood. Bioassays taken at 24 or 96 hours in either the cyclophosfamide (CP) or colchicine (COL) showed a significant increase in MN and BC (P values ranging from 0.0499 to 0.0036) compared with information from wild organisms collected over 3 years. Concentrationdependent and time-dependent responses support the proposal of using endemic Xenotoca melanosoma as a bioindicator of genotoxicity and cytotoxicity with a high transcendence for the health of the entire ecosystem and evaluation of the Lerma-Chapala watershed.