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Increasing the Fidelity of Noncanonical Amino Acid Incorporation in Cell-Free Protein Synthesis

Research paper by Qinglei Gan, Chenguang Fan

Indexed on: 04 Dec '16Published on: 02 Dec '16Published in: BBA General Subjects



Abstract

Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression.

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