In vivo bioluminescence imaging of labile iron pools in a murine model of sepsis with a highly selective probe.

Research paper by Ping P Feng, Lin L Ma, Feng F Xu, Xueyan X Gou, Lupei L Du, Bowen B Ke, Minyong M Li

Indexed on: 08 Mar '20Published on: 17 Jun '19Published in: Talanta


Iron plays an essential role in biological system. An approach for in vivo imaging of this metal ion is needed to investigate its complex contributions to physiological and pathological processes. Herein, we present a bioluminescent probe FP-1 as a powerful tool for targeting Fe detection in vitro and in vivo. The turn-on sensing scheme is based on the caged strategy of luciferin-luciferase system. FP-1 not only can detect accumulations of exogenous Fe in living animal, but also has the capability of monitoring labile endogenous Fe levels in animal model of sepsis. Implementation of this technique provides a valuable opportunity for understanding underlying mechanisms of Fe in biological processes and disease states. Copyright © 2019 Elsevier B.V. All rights reserved.