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In vitro translation.

Research paper by R R Jagus, B B Joshi, S S Miyamoto, G S GS Beckler

Indexed on: 30 Jan '08Published on: 30 Jan '08Published in: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]



Abstract

The two most frequently used systems for in vitro translation are the rabbit reticulocyte system and the wheat germ extract. These systems are useful for mRNAs isolated from cells, tissues, and capped or uncapped mRNA produced in vitro by transcription of cDNA. In a combined system, mRNA can be transcribed and translated in a single reaction. In addition these systems can be used for translation reactions with biotinylated amino acids; this allows capture of the newly synthesized protein using streptavidin immobilized on agarose.