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In vitro production of PLRV and PSTVd-free plants of potato using electrotherapy

Research paper by Balwinder Singh, Amritpal Kaur

Indexed on: 08 Jan '17Published on: 01 Dec '16Published in: Journal of Crop Science and Biotechnology



Abstract

Abstract Potato leafroll virus (PLRV) and Potato spindle tuber viroid (PSTVd) were responsible for major economic losses in potato production. Effect of electrotherapy on the regeneration response and production of PLRV and PSTVd-free plants from potato explants is reported. DAS-ELISA and RT-PCR were used for indexing of infected and in vitro-regenerated potato plants. Shoot tips, nodal segments, and tuber sprouts of infected potato plants were exposing to electric current intensities of 5- 25 mA in separate lots for a time period of 5-25 min and cultured on medium. Regeneration response was not significantly changed by exposing explants to 5-10 mA for 5-10 min. Electrotherapy at 15, 20, and 25 mA for 15-25 min had slightly decreased regeneration response of explants and current treatment at 20 and 25 mA for 20 min gave an appreciable number of PLRV and PSTVd-free plants. In a comparative study, electrotherapy of shoot tips and sprouts at 20 mA/20 min proved effective for knocking out virus and viroid without any adverse effecta on regeneration response and production of plantlets. Shoot tips gave 46.72% PLRV and 45.90% PSTVd-free plants with regeneration response of 84.71% and sprouts gave 42.85% PLRV and 43.60% PSTVd-free plants with regeneration response of 92.35% by using 20 mA/20 min treatment. Nodal segments showed less regeneration response (78.47%) and produced a low frequency of PLRV (34.51%) and PSTVd (33.62%)-free plants after this treatment. The protocol is simple and effective for eradicating PLRV and PSTVd from infected potato germplasm.Abstract Potato leafroll virus (PLRV) and Potato spindle tuber viroid (PSTVd) were responsible for major economic losses in potato production. Effect of electrotherapy on the regeneration response and production of PLRV and PSTVd-free plants from potato explants is reported. DAS-ELISA and RT-PCR were used for indexing of infected and in vitro-regenerated potato plants. Shoot tips, nodal segments, and tuber sprouts of infected potato plants were exposing to electric current intensities of 5- 25 mA in separate lots for a time period of 5-25 min and cultured on medium. Regeneration response was not significantly changed by exposing explants to 5-10 mA for 5-10 min. Electrotherapy at 15, 20, and 25 mA for 15-25 min had slightly decreased regeneration response of explants and current treatment at 20 and 25 mA for 20 min gave an appreciable number of PLRV and PSTVd-free plants. In a comparative study, electrotherapy of shoot tips and sprouts at 20 mA/20 min proved effective for knocking out virus and viroid without any adverse effecta on regeneration response and production of plantlets. Shoot tips gave 46.72% PLRV and 45.90% PSTVd-free plants with regeneration response of 84.71% and sprouts gave 42.85% PLRV and 43.60% PSTVd-free plants with regeneration response of 92.35% by using 20 mA/20 min treatment. Nodal segments showed less regeneration response (78.47%) and produced a low frequency of PLRV (34.51%) and PSTVd (33.62%)-free plants after this treatment. The protocol is simple and effective for eradicating PLRV and PSTVd from infected potato germplasm.Potato leafrollin vitro