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In vitro plantlet production system for Kaempferia galanga, a rare Indian medicinal herb

Research paper by Fatima Shirin, Sandeep Kumar, Yogeshwar Mishra

Indexed on: 01 Dec '00Published on: 01 Dec '00Published in: Plant Cell, Tissue and Organ Culture (PCTOC)



Abstract

A rapid clonal propagation system for Kaempferia galanga (Zingiberaceae), a rare folk medicinal herb has been developed. Various concentrations of 6-benzyladenine (BA) and a range of auxins have been investigated for in vitro plantlet production, using rhizomes as explants. In vitro plantlet production has been achieved on 0.75 × Murashige and Skoog (MS) medium supplemented with 12 μM BA, 3 μM ∝-naphthaleneacetic acid (NAA) and 3% sucrose. The procedure ensures 13-fold rate of plantlet production every 4 weeks. Hardened plantlets produced normal storage roots as the parent plants. Around 1,000 plantlets have been produced successfully for field transfer.