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In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae.

Research paper by X X De Jesús-Martínez, A A Olmedo-Juárez, J J Olivares-Pérez, A A Zamilpa, P P Mendoza de Gives, M E ME López-Arellano, S S Rojas-Hernández, A A Villa-Mancera, L M LM Camacho-Díaz, M M Cipriano-Salazar

Indexed on: 11 Jan '19Published on: 11 Jan '19Published in: BioMed research international



Abstract

The aim of this study was to evaluate the lethal effect of a methanolic extract (ME) from fruits against eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC and LC) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC = 78.38 and 0.00064 mg/mL and LC =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the fruit ME possesses ovicidal and larvicidal properties (gallotannins: methyl gallate) against that needs to be investigated more for the control of gastroenteric nematodes in ruminants.