In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs.

Research paper by Katherine J KJ Elliott, Satoru S Eguchi

Indexed on: 25 Jan '17Published on: 25 Jan '17Published in: Methods in molecular biology (Clifton, N.J.)

Abstract

Studying signal transduction in hypertension model systems in vitro will include several steps: (1) develop the cell culture model and induce hypertensive changes, (2) observe kinase activation, (3) manipulate signal transduction pathways, and (4) observe physiologic outputs. This chapter will provide the reader with overviews of the techniques our lab uses to inhibit signaling pathways with inhibitory RNAs and the outputs we use to monitor the effects.

In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs.

Abstract

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In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs.

Abstract

More like this:

In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation, and Assessment of Physiologic Outputs.

Signal transduction at the single-cell level: Approaches to study the dynamic nature of signaling networks.

Network regulation of calcium signal in stomatal development.

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