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Identification of hybrids in broad-leaved Potamogeton species (Potamogetonaceae) in China using nuclear and chloroplast DNA sequence data

Research paper by Zhi-Yuan Du, Chun-Feng Yang, Jin-Ming Chen, You-Hao Guo

Indexed on: 25 Apr '10Published on: 25 Apr '10Published in: Plant systematics and evolution = Entwicklungsgeschichte und Systematik der Pflanzen



Abstract

Hybridization is relatively frequent in the pondweed genus Potamogeton. A total of five putative hybrids of broad-leaved Potamogeton in China were collected in our recent investigations. We used internal transcribed spacers (ITS) of nuclear ribosomal DNA and chloroplast rbcL gene sequences to confirm the origins of the putative hybrids. Using ITS sequence additivity, we confirmed that the five putative hybrids were P. × anguillanus Koidzumi (P. wrightii × P. perfoliatus), P. × malainoides Miki (P. distinctus × P. wrightii), P. distinctus × P. nodosus, P. nodosus × P. wrightii, and P. distinctus × P.gramineus. The latter four hybrids are new records for China, and P. distinctus × P.gramineus is a new hybrid combination in Potamogeton. We found a new genotype of P. perfoliatus in northeast China. Hybrids between the new and a common genotype of P. perfoliatus were found in Central China. The maternal parents of the six hybrids were confirmed by chloroplast rbcL gene sequence data. The hybrids P. × anguillanus and P. distinctus × P.gramineus are reciprocal hybrids. P. × anguillanus has multiple origins from different populations. P. distinctus × P.gramineus has multiple origins within a single population.