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Human adenoviruses: propagation, purification, quantification, and storage.

Research paper by Maurice M Green, Paul M PM Loewenstein

Indexed on: 05 Sep '08Published on: 05 Sep '08Published in: Current protocols in microbiology



Abstract

Detailed protocols are described for the propagation of adenoviruses (Ads) and adenovirus (Ad) vectors and their purification by CsCl equilibrium density gradient centrifugation. A discussion of monolayer and spinner cell culture techniques suitable, respectively, for small- and large-scale growth of adenoviruses is provided. Protocols for cloning into and growth of Ad replication-deficient vectors using a convenient commercially available system are described. Lastly, time-tested plaque titration protocols for the accurate and convenient measurement of the infectivity of adenoviruses and adenovirus vectors are provided in detail.