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Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes.

Research paper by Minh Nguyen MN Nguyet, Thi Hue Kien TH Duong, Vu Tuan VT Trung, Than Ha Quyen TH Nguyen, Chau N B CN Tran, Vo Thi VT Long, Le Thi le T Dui, Hoa Lan HL Nguyen, Jeremy J JJ Farrar, Edward C EC Holmes, Maia A MA Rabaa, Juliet E JE Bryant, Truong Thanh TT Nguyen, Huong Thi Cam HT Nguyen, Lan Thi Hong LT Nguyen, et al.

Indexed on: 16 May '13Published on: 16 May '13Published in: PNAS



Abstract

Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1-4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.