Heparin is biocompatible and can induce differentiation of human dental pulp cells.

Research paper by E M EM Rodrigues, A L G ALG Cornélio, P H PH Godoi, P I PI da Costa, C C Rossa-Junior, G G Faria, J M JM Guerreiro Tanomaru, M M Tanomaru-Filho

Indexed on: 20 Dec '18Published on: 20 Dec '18Published in: International Endodontic Journal


To investigate the biocompatibility, osteogenic bioactivity, and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). hDPCs were exposed to the heparin and cell viability was assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay and the detection of calcium deposits by Alizarin Red Staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t test (α = 0.05). Heparin had no cytotoxic effect and did not induce apoptosis. After three days, heparin had significantly higher ALP activity in comparison to the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expression was significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.