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Genotoxicity and pharmacokinetic characterization of Cereus jamacaru ethanolic extract in rats.

Research paper by Iris U IU Medeiros, Rhoza A RA Medeiros, Raul Hernandes RH Bortolin, Fernando Márlisson FM Queiroz, Vivian Nogueira VN Silbiger, Stephen S Pflugmacherd, Aline A Schwarz

Indexed on: 20 Dec '18Published on: 20 Dec '18Published in: Bioscience Reports



Abstract

The effect of ethanolic extract in rats was analyzed using genotoxicity assays and liver and gene expression. The lyophilized extract of cladodes was analyzed with LC-MS/MS. Male Wistar rats (n=36) were equally distributed into six groups that did (+) or did not (-) receive cyclophosphamide treatments: Control (-); Control (+); EXP 1 (-) and EXP 1 (+), both treated with 210 mg/kg of ethanolic extract; and EXP 2 (-) and EXP 2 (+), both treated with 420 mg/kg of ethanolic extract. After 30 days of treatment, body weight and food and water intake were monitored. Right femur of the rats and spinal canal fluid were harvested and used for genotoxicity assays, and the liver samples were used for gene expression studies. The phytochemical analysis identified novels compounds. Animals treated with showed lower body weight and food ingestion compared to controls (p<0.05). The genotoxicity assay showed an absence of ethanolic extract cytotoxicity. expression was higher in EXP 2 groups compared to EXP 1 (-) group (p<0.05). expression was higher in negative groups compared to the positive groups. These results indicated a new phytochemical characterization of and its effect on food ingestion and body weight gain. Moreover, the genotoxicity assay suggested ethanolic extract treatment present significant intrinsic genotoxic potential and the enhanced expression of and on extract treatment suggests a role of the efflux transporter and microsomal enzyme, respectively, in pharmacokinetics. ©2018 The Author(s).