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Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.

Research paper by Kamalaveni R KR Prabakar, Juan J Domínguez-Bendala, R Damaris RD Molano, Antonello A Pileggi, Susana S Villate, Camillo C Ricordi, Luca L Inverardi

Indexed on: 27 Dec '11Published on: 27 Dec '11Published in: Cell transplantation



Abstract

We sought to assess the potential of human cord blood-derived mesenchymal stem cells (CB-MSCs) to derive insulin-producing, glucose-responsive cells. We show here that differentiation protocols based on stepwise culture conditions initially described for human embryonic stem cells (hESCs) lead to differentiation of cord blood-derived precursors towards a pancreatic endocrine phenotype, as assessed by marker expression and in vitro glucose-regulated insulin secretion. Transplantation of these cells in immune-deficient animals shows human C-peptide production in response to a glucose challenge. These data suggest that human cord blood may be a promising source for regenerative medicine approaches for the treatment of diabetes mellitus.