Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae.

Research paper by Richard R Haindl, Abigail J AJ Deloria, Caterina C Sturtzel, Harald H Sattmann, Wolfgang W Rohringer, Balthasar B Fischer, Marco M Andreana, Angelika A Unterhuber, Thorsten T Schwerte, Martin M Distel, Wolfgang W Drexler, Rainer R Leitgeb, Mengyang M Liu

Indexed on: 29 Apr '20Published on: 29 Apr '20Published in: Biomedical optics express


We present a dual modality functional optical coherence tomography and photoacoustic microscopy (OCT-PAM) system. The photoacoustic modality employs an akinetic optical sensor with a large imaging window. This imaging window enables direct reflection mode operation, and a seamless integration of optical coherence tomography (OCT) as a second imaging modality. Functional extensions to the OCT-PAM system include Doppler OCT (DOCT) and spectroscopic PAM (sPAM). This functional and non-invasive imaging system is applied to image zebrafish larvae, demonstrating its capability to extract both morphological and hemodynamic parameters in small animals, which are essential and critical in preclinical imaging for physiological, pathophysiological and drug response studies. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.