Fluorescence microscopy study of Rap1 subcellular localization.

Research paper by Luca L Goitre, Valentina V Cutano, Saverio Francesco SF Retta

Indexed on: 29 Jan '14Published on: 29 Jan '14Published in: Methods in molecular biology (Clifton, N.J.)


The Ras-related GTPase Rap has been implicated in multiple cellular functions. In particular, Rap1 is a crucial regulator of both inside-out integrin activation and outside-in E-cadherin-mediated signaling. Thus, Rap1 was proposed as a fundamental regulator of the cross talk between cadherins and integrins. We provide microscopic techniques to study subcellular localization of Rap1 protein in the crosstalk between integrins and cadherins.