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Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations.

Research paper by Artur G AG Leme Silva, Maíra H MH Nagai, Bettina B Malnic

Indexed on: 10 Jun '18Published on: 10 Jun '18Published in: Methods in molecular biology (Clifton, N.J.)



Abstract

The mouse olfactory epithelium is composed of a heterogeneous population of olfactory sensory neurons, where each neuron expresses one single type of odorant receptor gene, out of a repertoire of ~1000 different genes. Fluorescent-activated cell sorting (FACS) is a powerful technique, which can be used to isolate a cellular subpopulation from a heterogeneous tissue. The sorted neurons can then be used in gene expression studies, or analyzed for the presence of different DNA epigenetic modification marks. Here we describe a method to separate a subpopulation of olfactory sensory neurons expressing the odorant receptor Olfr17. In this method, the main olfactory epithelium from transgenic Olfr17-IRES-GFP mice is dissociated into single cells, followed by separation of the GFP positive cells by FACS.