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Extracellular Ligninolytic Enzymes in Bjerkandera adusta and Lentinus squarrosulus.

Research paper by Astha A Tripathi, R C RC Upadhyay, Surendra S Singh

Indexed on: 03 Sep '13Published on: 03 Sep '13Published in: Indian Journal of Microbiology



Abstract

Extracellular ligninolytic enzyme activities were determined in two white-rot fungi, Bjerkandera adusta and Lentinus squarrosulus. To investigate the activity of extracellular enzymes, cultures were incubated over a period of 20 days in nutrient rich medium (NRM) and nutrient poor medium under static and shaking conditions. Enzymatic activity was varied with media and their incubation conditions. The highest level of Aryl alcohol oxidase (AAO) was detected under shaking condition of both medium while Manganese peroxidase (MnP) activity was best in NRM under both conditions. AAO is the main oxidases enzyme in B. adusta while laccase plays important role in L. squarrosulus. MnP is the main peroxidase enzyme in both varieties.