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Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones.

Research paper by Dengfeng D Yang, Takahiro T Mori, Takashi T Matsui, Makoto M Hashimoto, Hiroyuki H Morita, Isao I Fujii, Ikuro I Abe

Indexed on: 11 Jun '14Published on: 11 Jun '14Published in: Acta Crystallographica Section F



Abstract

CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 Å, β=114.4°.