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Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli

Research paper by Ziyu Dai, Brian S. Hooker, Ryan D. Quesenberry, Jianwei Gao

Indexed on: 01 Mar '99Published on: 01 Mar '99Published in: Applied Biochemistry and Biotechnology



Abstract

Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 μmol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 μmol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively, Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.