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Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae

Research paper by Hee-Seo Kim, Dong Whan Lee, Eun Ja Ryu, Tai-Boong Uhm, Moon-Sik Yang, Jung Bae Kim, Keon-Sang Chae

Indexed on: 01 Jul '99Published on: 01 Jul '99Published in: Biotechnology Letters



Abstract

The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase preparation free of an exoinulinase and an extracellular invertase in the culture medium. A recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony, when inulin was included in the medium.