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Expression of human estrogen receptors in transgenic mice

Imported: 25 Feb '17 | Published: 01 Jul '03

Jeffrey M. Arbeit, Peter J. Kushner, Rosalie M. Uht, Birgit Anderegg

USPTO - Utility Patents

Abstract

The present invention relates to improved compositions and methods for assaying the efficacy of novel drugs functioning either as agonists or antagonists at nuclear receptors. In particular, the invention provides transgenic non-human animals comprising a human steroid hormone receptor gene operably linked to a promoter which directs expression of the steroid hormone receptor gene in an epithelial cell of the non-human animals.

Claims

1. A transgenic mouse whose genome comprises a human estrogen receptor gene operably linked to a human keratin-14 promoter, whereby expression of the human estrogen receptor gene in an epithelial cell causes a phenotype selected from the group consisting of epidermal hyperplasia and erythematous ears.

2. The transgenic mouse of claim 1, wherein said expression of the human estrogen receptor gene causes epidermal hyperplasia.

3. The transgenic mouse of claim 1, wherein said expression of the human estrogen receptor gene causes erythematous ears.

4. A method of testing a composition for the ability to modulate the activity of a human estrogen receptor, the method comprising:

5. The method of claim 4, wherein expression of the human estrogen receptor gene causes epidermal hyperplasia.

6. The method of claim 4, wherein expression of the human estrogen receptor gene causes erythematous ears.

7. The method of claim 4, wherein said composition is administered topically.

8. The method of claim 4, wherein said composition is administered orally.

9. The method of claim 4, wherein said composition is administered parenterally.

10. The method of claim 4, wherein said changes are detected by visual inspection.

11. The method of claim 4, wherein said changes are detected by microscopic observation.