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Exploring the Galleria mellonella model to study antifungal photodynamic therapy.

Research paper by Lívia Mara Alves LMA Figueiredo-Godoi, Raquel Telles RT Menezes, Jéssica Shiotani JS Carvalho, Maíra Terra MT Garcia, Aguinaldo Garcez AG Segundo, Antonio Olavo Cardoso AOC Jorge, Juliana Campos JC Junqueira

Indexed on: 19 Nov '19Published on: 18 May '19Published in: Photodiagnosis and Photodynamic Therapy



Abstract

Antimicrobial photodynamic therapy (aPDT) shows antimicrobial activity on yeast of the genus Candida. In photodynamic therapy, the depth at which the light penetrates the tissue is extremely important for the elaboration of the treatment. The aim of this study was to evaluate the action of aPDT on experimental candidiasis and the laser impact in the tissue using Galleria mellonella as the infection model. G. mellonella larvae were infected with different Candida albicans strains. After 30 min, they were treated with methylene blue-mediated aPDT and a low intensity laser (660 nm). The larvae were incubated at 37 °C for seven days and monitored daily to determine the survival curve, using the Log-rank test (Mantel Cox). To evaluate the distribution of the laser as well as its depth of action in the larva body, the Interactive 3D surface PLOT of Image J was used. The effects of aPDT on the immune system were also evaluated by the quantification of hemocytes in the hemolymph of G. mellonella after 6 hours of Candida infection (ANOVA and Tukey's test). In both the ATCC 18804 strain and the C. albicans clinical strain 17, aPDT prolonged the survival of the infected G. mellonella larvae by a lethal fungal dose. There was a statistically significant difference between the aPDT and the control groups in the ATCC strain (P = 0.0056). The depth of laser action in the insect body without the photosensitizer was 2.5 mm and 2.4 mm from the cuticle of the larva with the photosensitizer. In the larvae, a uniform distribution of light occurred along 32% of the body length for the group without the photosensitizer and in 39.5% for the group with the photosensitizer. In the immunological analysis, the infection by C. albicans ATCC 18804 in G. mellonella led to a reduction in the number of hemocytes in the hemolymph. The aPDT and laser treatment induced a slight increase in the number of hemocytes. Both aPDT and laser treatment positively influenced the treatment of experimental candidiasis. G. mellonella larvae were a useful model for the study of light tissue penetration in photodynamic therapy. Copyright © 2019. Published by Elsevier B.V.

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