Indexed on: 21 Mar '17Published on: 21 Mar '17Published in: Journal of Agricultural and Food Chemistry
Eugenol-loaded nanoemulsion by zein and sodium caseinate (NaCas) was prepared without using specific equipment or organic solvents. The deprotonated eugenol in hot alkaline was added into NaCas/zein mixtures with different mass ratios at pH 11.5, and then neutralized to pH 7.0. The nanoemulsions showed well-defined diameter (around 109-139 nm) and negative surface potential (-28.5~ -35.8 mv). The entrapment efficiency (EE) of 1% v/v eugenol reached 84.24% by 2% m/v NaCas/zein at a mass ratio of 1:1. This formulation also showed the narrowest size distribution, extraordinary stability during ambient storage (22 oC) up to 30 days, and retained good re-dispersiblity after spray- or freeze- drying. Current study showed a promising green strategy to delivery lipophilic compounds containing hydroxyl group.