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Enterobacter muelleri sp. nov., isolated from the rhizosphere of Zea mays.

Research paper by Peter P Kämpfer, John A JA McInroy, Stefanie P SP Glaeser

Indexed on: 22 Aug '15Published on: 22 Aug '15Published in: International journal of systematic and evolutionary microbiology



Abstract

A beige-pigmented, oxidase-negative bacterial strain (JM-458T), isolated from a rhizosphere sample was studied in a polyphasic taxonomic approach. Cells of the isolate were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain JM-458T with sequences of type strains of most closely related Enterobacter species showed highest sequence similarities to Enterobacter mori (98.7%), Enterobacter hormaechei (98.3%), Enterobacter cloacae subsp. dissolvens, Enterobacter ludwigii and Enterobacter asburiae (all 98.2%). 16S rRNA gene sequence similarities to all other Enterobacter species were below 98%. Multilocus sequence analyses (MLSA) based on concatenated partial rpoB, gyrB, infB and atpD sequences showed a clear distinction of strain JM-458T from the closest related type strains. The fatty acid profile of the strain consisted of the major fatty acids C16:0 ,C17:0 cyclo, C15:0 iso 2-OH/ C16:1 ω7c, and C18:1 ω7c. DNA-DNA hybridizations between JM-458T and type strains of E. mori, E. hormaechei and E. ludwigii resulted in similarity values of 29% (reciprocal 25%), 24% (reciprocal 43%), and 16% (reciprocal 17%), respectively. DNA-DNA hybridization results together with MLSA results and the differentiating biochemical and chemotaxonomic properties showed, that strain JM-458T represent a novel Enterobacter species, for which the name Enterobacter muelleri sp. nov. (type strain JM-458T = DSM 29346T = CIP 110826T = LMG 28480T = CCM 8546T), is proposed.