Indexed on: 20 Dec '18Published on: 20 Dec '18Published in: Clinical hemorheology and microcirculation
The formation of a functionally-confluent endothelial cell (EC) monolayer affords proliferation of EC, which only happens in case of appropriate migratory activity. The migratory pathway of human umbilical endothelial cells (HUVEC) was investigated on different polymeric substrates. Surface characterization of the polymers was performed by contact angle measurements and atomic force microscopy under wet conditions. 30,000 HUVEC per well were seeded on polytetrafluoroethylene PTFE (θadv = 119°±2°), on low-attachment dish LAP (θadv = 28°±2°) and on polystyrene based tissue culture plates (TCP, θadv = 22°±1°). HUVEC tracks (trajectories) were recorded by time lapse microscopy and the euclidean distance (straight line between starting and end point), the total distance and the velocity of HUVEC not leaving the vision field migration were determined. On PTFE, 42 HUVEC were in the vision field directly after seeding. The mean length of single migration steps (SML) was 6.1±5.2μm, the mean velocity (MV) 0.40±0.3μm·min - 1 and the complete length of the trajectory (LT) was 710±440μm. On TCP 82 HUVEC were in the vision field subsequently after seeding. The LT was 840±550μm, the SML 6.1±5.2μm and the MV 0.44±0.3μm·min - 1. The trajectories on LAP differed significantly in respect to SML (2.4±3.9μm, p < 0.05), the MV (0.16±0.3μm·min - 1, p < 0.05) and the LT (410±300μm, p < 0.05), compared to PTFE and TCP. Solely on TCP a nearly confluent EC monolayer developed after three days. While on TCP diffuse signals of vinculin were found over the whole basal cell surface organizing the binding of the cells by focal adhesions, on PTFE vinculin was merely arranged at the cell rims and on the hydrophilic material (LAP) no focal adhesions were found. The study revealed that the wettability of polymers affected not only the initial adherence but also the migration of EC, which is of importance for the proliferation and ultimately the endothelialization of polymer-based biomaterials.