Effects of parvovirus B19 in vitro infection on monocytes from systemic sclerosis patients: enhanced inflammatory pathways by caspase-1 activation and cytokine production

Research paper by Krystyna Zakrzewska, Rosaria Arvia, Maria Gabriella Torcia, Ann Maria Clemente, Michele Tanturli, Giuseppe Castronovo, Gianluca Sighinolfi, Dilia Giuggioli, Clodoveo Ferri

Indexed on: 12 Apr '19Published on: 09 Apr '19Published in: Journal of Investigative Dermatology


Parvovirus B19 (B19V) has been proposed as triggering agent of some autoimmune diseases including systemic sclerosis (SSc). In this study, we investigated whether B19V infection in vitro differently activates inflammatory pathways, including those dependent on caspase-1 activation, in monocytes from SSc patients and healthy controls.We showed that B19V can infect both THP-1 cells and primary monocytes but is not able to replicate in these cells. B19V infection increases TNFα production and induces NLRP3-mediated caspase-1 activation in both PMA-differentiated THP-1 cells and in monocytes from SSc patients but not from healthy controls.