Indexed on: 20 Jan '09Published on: 20 Jan '09Published in: Methods in molecular biology (Clifton, N.J.)
It is sometimes necessary to assess the genetic relatedness of isolates to identify the origin of an infection. In addition, evidence is accumulating that drug resistance can be associated with strains from a particular clade and that strains can exhibit anatomical specificity. It may, therefore, be valuable in the near future to screen for strains with a propensity for drug resistance. While a number of methods exist for genetically fingerprinting the infectious fungi, only a few provide the necessary resolution not only for distinguishing whether strains are highly related or unrelated, but also for grouping a strain in a particular clade. Here, we provide the procedures for performing the two methods that have proven most effective in the past 5 years: Southern blot hybridization of restriction fragments with complex probes and multilocus sequence typing (MLST).