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DNA-binding geometry dependent energy transfer from 4',6-diamidino-2-phenylindole to cationic porphyrins.

Research paper by Biao B Jin, Kil Sik KS Min, Sung Wook SW Han, Seog K SK Kim

Indexed on: 07 Jul '09Published on: 07 Jul '09Published in: Biophysical Chemistry



Abstract

The circular and linear dichroism (CD and LD) spectral properties of the meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP)-DNA complex at a [porphyrin]/[DNA] ratio below 0.015 showed that TMPyP intercalates between DNA base pairs. Contrarily, when cis-bis(N-methylpyridinium-4-yl)porphyrin (BMPyP) is associated with DNA, no CD spectrum was induced and a bisignate LD spectrum was observed. These spectral properties of both the TMPyP and BMPyP were essentially retained when the minor groove of the DNA was saturated with 4',6-diamidino-2-phenylindole (DAPI). The fluorescence of the DNA-bound DAPI was effectively quenched by BMPyP and TMPyP. The quenching by BMPyP can be described through a pure static mechanism while TMPyP quenching produced an upward bending curve in the Stern-Volmer plot. Quenching efficiency was by far greater than predicted by the "sphere of action model", suggesting that the DNA provides some additional processes for an effective energy transfer.