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Differentiation of mesenchymal stem cell in the microenviroment of retinitis pigmentosa.

Research paper by Dong-Mei DM Huo, Fang-Tian FT Dong, Wei-Hong WH Yu, Fei F Gao

Indexed on: 01 Jan '10Published on: 01 Jan '10Published in: International journal of ophthalmology



Abstract

CORRESPONDENCE TO: Fang-Tian Dong. Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China. d_fangtian@sina.comTo access the differentiation of rat mesenchymal stem cell (MSC) in the microenvironment of retinal degeneration induced by the administration of sodium iodate.In-vitro cultured Lewis rat MSC were injected into the sub-retinal space of NaIO(3) induced retinal degeneration rat eyes (30g/L NaIO(3) 100mg/kg). To observe the trace and differentiation of MSC by immuno-fluorescent method successively in 5 weeks after the surgery.The majority of the transplanted cells stay in retinal pigment epithelium layer and cones & rods layer. From the 2(nd) week after transplantation, the engrafted MSC express PCK and rhodopsin under fluorescent microscope.MSC can survive mainly in the outer layer of retina in the microenvironment of retinal degeneration and differentiate forward the RPE cell and photoreceptor.