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Developmental toxicity assay using high content screening of zebrafish embryos.

Research paper by Susan S Lantz-McPeak, Xiaoqing X Guo, Elvis E Cuevas, Melanie M Dumas, Glenn D GD Newport, Syed F SF Ali, Merle G MG Paule, Jyotshna J Kanungo

Indexed on: 30 May '14Published on: 30 May '14Published in: Journal of Applied Toxicology



Abstract

Typically, time-consuming standard toxicological assays using the zebrafish (Danio rerio) embryo model evaluate mortality and teratogenicity after exposure during the first 2 days post-fertilization. Here we describe an automated image-based high content screening (HCS) assay to identify the teratogenic/embryotoxic potential of compounds in zebrafish embryos in vivo. Automated image acquisition was performed using a high content microscope system. Further automated analysis of embryo length, as a statistically quantifiable endpoint of toxicity, was performed on images post-acquisition. The biological effects of ethanol, nicotine, ketamine, caffeine, dimethyl sulfoxide and temperature on zebrafish embryos were assessed. This automated developmental toxicity assay, based on a growth-retardation endpoint should be suitable for evaluating the effects of potential teratogens and developmental toxicants in a high throughput manner. This approach can significantly expedite the screening of potential teratogens and developmental toxicants, thereby improving the current risk assessment process by decreasing analysis time and required resources.