Development and Application of a Liquid Preparation with Oil-Oxidizing Bacteria

Research paper by V. A. Chugunov, Z. M. Ermolenko, S. K. Zhigletsova, I. I. Martovetskaya, R. I. Mironova, N. A. Zhirkova, V. P. Kholodenko, N. N. Urakov

Indexed on: 01 Nov '00Published on: 01 Nov '00Published in: Applied Biochemistry and Microbiology


An association of four bacterial strains with high oil-oxidizing and bioemulsifying activities, psychrophilicity, resistance to chemical pollutants, and lack of pathogenicity was selected from a collection of natural oil-oxidizing microorganisms. A new liquid preparation containing stabilizers and preservatives that maintain cell viability and oil-oxidizing activity during long-term storage was developed. A field experiment in oil-polluted sod-podzol and clay sand soils demonstrated that this preparation accelerated the biodegradation of oil and its individual fractions, especially in the presence of mineral and organic fertilizers. Treatment of oil-polluted soil with this preparation and additives decreased the oil-induced suppression of certain groups of soil microflora.