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Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag.

Research paper by Rui R Chen, Steven W SW Plouffe, Kun-Liang KL Guan

Indexed on: 20 Dec '18Published on: 20 Dec '18Published in: Methods in molecular biology (Clifton, N.J.)



Abstract

Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag technology has been developed to detect protein phosphorylation. Here, we describe our method for using Phos-tag gels to compare the phosphorylation status of YAP and TAZ, the most important downstream effectors of the Hippo pathway.