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Detection of IFN-α Produced in the Presence of Plasma γ-Globulin Fraction Proteins

Research paper by S. B. Cheknyov, A. A. Babayants, I. E. Efremova, E. N. Yushkovets

Indexed on: 17 Sep '09Published on: 17 Sep '09Published in: Bulletin of Experimental Biology and Medicine



Abstract

Interferon-α was detected in IFN pool produced by human leukocytes in the presence of γ-globulin fraction proteins, copper and zinc cations, and metal-modified γ-globulins. The cytokine appeared in culture medium at early terms (24 h) of incubation, is characterized by acid resistance, and is neutralized by antibodies to IFN-α. The content of IFN-α in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum γ-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation.