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Der lichtmikroskopische Nachweis der Arylsulfatase mit 8-Oxychinolinsulfat

Research paper by H. Woohsmann, W. Hartrodt

Indexed on: 01 Mar '67Published on: 01 Mar '67Published in: Histochemie. Histochemistry. Histochimie



Abstract

A method is described for the cytochemical demonstration of B-sulphatase. Fixation: Baker-formalin followed by immersing the material into Holt's sucrose solution. Incubation medium: 50 mg 8-Oxichinolinsulphate, 200 mg NaCl, 10 ml Michaelis buffer pH 5.8–6.0, 0.5 ml hex. Pararosanilin. The enzyme is demonstrable in the lysosomes of various organes. The preparation of the substrate is described.