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Cortical glial fibrillary acidic protein-positive cells generate neurons after perinatal hypoxic injury.

Research paper by Baoyuan B Bi, Natalina N Salmaso, Mila M Komitova, Maria V MV Simonini, John J Silbereis, Elise E Cheng, Janice J Kim, Suzannah S Luft, Laura R LR Ment, Tamas L TL Horvath, Michael L ML Schwartz, Flora M FM Vaccarino

Indexed on: 24 Jun '11Published on: 24 Jun '11Published in: The Journal of neuroscience : the official journal of the Society for Neuroscience



Abstract

Glial fibrillary acidic protein-positive (GFAP(+)) cells give rise to new neurons in the neurogenic niches; whether they are able to generate neurons in the cortical parenchyma is not known. Here, we use genetic fate mapping to examine the progeny of GFAP(+) cells after postnatal hypoxia, a model for the brain injury observed in premature children. After hypoxia, immature cortical astroglia underwent a shift toward neuronal fate and generated cortical excitatory neurons that appeared synaptically integrated into the circuitry. Fate-mapped cortical GFAP(+) cells derived ex vivo from hypoxic, but not normoxic, mice were able to form pluripotent, long-term self-renewing neurospheres. Similarly, exposure to low oxygen conditions in vitro induced stem-cell-like potential in immature cortical GFAP(+) cells. Our data support the conclusion that hypoxia promotes pluripotency in GFAP(+) cells in the cortical parenchyma. Such plasticity possibly explains the cognitive recovery found in some preterm children.