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Construction of neocentromere-based human minichromosomes for gene delivery and centromere studies.

Research paper by L H LH Wong, R R Saffery, K H A KH Choo

Indexed on: 29 May '02Published on: 29 May '02Published in: Gene Therapy



Abstract

Human neocentromeres are fully functional centromeres that arise naturally in non-centromeric regions devoid of alpha-satellite DNA. We have successfully produced a series of minichromosomes by telomere-associated truncation of a marker chromosome mardel(10) containing a neocentromere. The resulting minichromosomes are either linear or circular in nature, and range in size from approximately 650 kb to 2 Mb. These minichromosomes exhibit full centromeric activity, bind to essential centromere proteins, and are mitotically stable over many generations. They provide a useful system for dissecting the functional domains of complex eukaryotic centromeres and as vectors for therapeutic gene delivery.