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Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs.

Research paper by Maribel M Casas, Sonia S Pina, Bibiana B Peralta, Enric E Mateu, Jordi J Casal, Marga M Martín

Indexed on: 04 Nov '10Published on: 04 Nov '10Published in: The Veterinary Journal



Abstract

Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R(2)) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs.