Comparison of drug delivery properties of PEG-b-pdhpc micelles with different compositions

Research paper by Chun-yan Long, Ming-ming Sheng, Bin He, Yao Wu, Gang Wang, Zhong-wei Gu

Indexed on: 18 Feb '12Published on: 18 Feb '12Published in: Chinese Journal of Polymer Science


An anti-tumor drug doxorubicin was encapsulated in micelles of poly(ethylene glycol)-b-poly(2,2-dihydroxyl-methyl propylene carbonate) (PEG-b-PDHPC) diblock copolymers. The morphology of both blank micelles and drug loaded micelles was characterized by TEM. The in vitro drug release profiles of micelles were investigated. The cytotoxicity of the micelles was evaluated by incubating with Hela tumor cells and 3T3 fibroblasts. The drug loaded micelles were co-cultured with HepG2 cells to evaluate the in vitro anti-tumor efficacies. The results showed that the mean sizes of both micelles with different copolymer compositions increased after being loaded with drugs. The drug release rate of PEG45-b-PDHPC34 micelles was faster than that of mPEG114-b-PDHPC26 micelles. Both of the two block copolymers were non-toxic. The confocal laser scanning microscopy and flow cytometry results showed that both the drug loaded micelles could be internalized efficiently in HepG2 cells. The PEG45-b-PDHPC34 micelles exhibited higher anti-tumor activity comparing to mPEG114-b-PDHPC26 micelles.