Comparison of bacterial and phage display peptide libraries in search of target-binding motif

Research paper by Mojca Lunder, Tomaž Bratkovič, Bojan Doljak, Samo Kreft, Uroš Urleb, Borut Štrukelj, Nadja Plazar

Indexed on: 01 Nov '05Published on: 01 Nov '05Published in: Applied Biochemistry and Biotechnology


Genetic engineering allows modification of bacterial and bacteriophage genes, which code for surface proteins, enabling display of random peptides on the surface of these microbial vectors. Biologic peptide libraries thus formed are used for high-throughput screening of clones bearing peptides with high affinity for target proteins. There are reports of many successful affinity selections performed with phage display libraries and substantially fewer cases describing the use of bacterial display systems. In theory, bacterial display has some advantages over phage display, but the two systems have never been experimentally compared. We tested both techniques in selecting streptavidin-binding peptides from two commercially available libraries. Under similar conditions, selection of phage-displayed peptides to model protein streptavidin proved convincingly better.