Indexed on: 01 Jan '85Published on: 01 Jan '85Published in: Journal of the American Oil Chemists' Society
Soybean seeds were extracted with chloroform-methanol (2:1), methylene chloride-methanol (2:1) and hexane-isopropanol (3:2) mixtures. The seed lipids were then fractionated by column chromatography. Neutral lipids were further separated by thin layer chromatography (TLC) and quantified by acid charring method. Fatty acid methyl esters were prepared and analyzed by gas liquid chromatography (GLC). Our results show that methylene chloride-methanol (2:1) was as good as chloroform-methanol (2:1) for the extraction of soybean lipids, but hexane-isopropanol (3:2) was somewhat inferior.