Comparative studies of three solvent mixtures for the extraction of soybean lipids

Research paper by H. T. Khor, S. L. Chan

Indexed on: 01 Jan '85Published on: 01 Jan '85Published in: Journal of the American Oil Chemists' Society


Soybean seeds were extracted with chloroform-methanol (2:1), methylene chloride-methanol (2:1) and hexane-isopropanol (3:2) mixtures. The seed lipids were then fractionated by column chromatography. Neutral lipids were further separated by thin layer chromatography (TLC) and quantified by acid charring method. Fatty acid methyl esters were prepared and analyzed by gas liquid chromatography (GLC). Our results show that methylene chloride-methanol (2:1) was as good as chloroform-methanol (2:1) for the extraction of soybean lipids, but hexane-isopropanol (3:2) was somewhat inferior.