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Comparative Studies of Different Extracts from Eucommia ulmoides Oliv. against Rheumatoid Arthritis in CIA Rats.

Research paper by Jian-Ying JY Wang, Xiao-Jun XJ Chen, Lei L Zhang, Ying-Yi YY Pan, Zu-Xi ZX Gu, Shi-Min SM He, Zhe-Ping ZP Song, Ying Y Yuan

Indexed on: 15 Aug '18Published on: 15 Aug '18Published in: Evidence-based complementary and alternative medicine : eCAM



Abstract

To compare efficacy of different extracts from Oliv. with both immune inflammation and joint destruction in collagen induced arthritis (CIA) rat model. Rats were divided into normal group (Nor), control group (CIA), TG group (treated with tripterygium glycoside), E70 group (treated with 70% ethanol extract from Oliv.), EA group (treated with ethyl acetate fraction from E70), and EN group (treated with n-butyl alcohol fraction from E70). All extracts from Oliv. could significantly inhibit ankle swelling, pathological manifestations, and cytokine levels in serum and spleen, by using foot volume measurement, H&E staining, ELISA, and RT-QPCR methods, respectively. All extracts could significantly inhibit rough joint surface and marginal osteophytes, improve RANKL/OPG ratio, and decrease MMP-9 expression, by using micro-CT and immunohistochemical staining. The activation of IKK/NF-B signaling pathway was also inhibited by all extracts. In addition, ethyl acetate fraction from E70 presented better effect on RANKL/OPG system. This study identified effective extracts from Oliv. relieving immune inflammation and maintaining structural integrity of joints in CIA rats.

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