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Cocirculation of Plasmodium and Bacterial Deoxyribonucleic Acids in Blood of Febrile and Afebrile Children from Urban and Rural Areas in Gabon.

Research paper by Gaël G Mourembou, Sydney Maghendji SM Nzondo, Angélique A Ndjoyi-Mbiguino, Jean Bernard JB Lekana-Douki, Lady Charlène LC Kouna, Pierre Blaise PB Matsiegui, Rella Zoleko RZ Manego, Irene Pegha IP Moukandja, Alpha Kabinet AK Keïta, Hervé H Tissot-Dupont, Florence F Fenollar, Didier D Raoult

Indexed on: 27 Apr '16Published on: 27 Apr '16Published in: The American journal of tropical medicine and hygiene



Abstract

Malaria is considered to be the most common etiology of fever in sub-Saharan Africa while bacteremias exist but are under assessed. This study aimed to assess bacteremias and malaria in children from urban and rural areas in Gabon. DNA extracts from blood samples of 410 febrile and 60 afebrile children were analyzed using quantitative polymerase chain reaction. Plasmodium spp. was the microorganism most frequently detected in febrile (78.8%, 323/410) and afebrile (13.3%, 8/60) children, (P < 0.001). DNA from one or several bacteria were detected in 17 febrile patients (3.7%) but not in the controls (P = 0.1). This DNA was more frequently detected as coinfections among febrile children testing positive for Plasmodium (4.6%, 15/323) than in those testing negative for Plasmodium (0%, 0/87; P = 0.04). The bacteria detected were Streptococcus pneumoniae 2.4% (10/410), Staphylococcus aureus 1.7% (7/410), Salmonella spp. 0.7% (3/410), Streptococcus pyogenes 0.2% (1/410) and Tropheryma whipplei 0.2% (1/410) only in febrile children. Coxiella burnetii, Borrelia spp., Bartonella spp., Leptospira spp., and Mycobacterium tuberculosis were not observed. This paper reports the first detection of bacteremia related to T. whipplei in Gabon and shows that malaria decreases in urban areas but not in rural areas. Coinfections in febrile patients are common, highlighting the need to improve fever management strategies in Gabon.

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