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Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii.

Research paper by Deborah M DM Waters, Patrick G PG Murray, Yuta Y Miki, Angel T AT Martínez, Maria G MG Tuohy, Craig B CB Faulds

Indexed on: 13 Mar '12Published on: 13 Mar '12Published in: Applied and environmental microbiology



Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.