Indexed on: 19 Oct '17Published on: 19 Oct '17Published in: Journal of Global Antimicrobial Resistance
The aim of this study was to investigate carbapenemase-producing Enterobacteriaceae (CPE) in companion animals.Between October 2015 and April 2016, 533 rectal swabs were obtained from healthy and diseased pets in different cities in Algeria. Samples collected were plated on MacConkey agar supplemented with ertapenem (0.5mg/L). The isolates were identified by MALDI-TOF mass spectroscopy. Antimicrobial susceptibility testing was performed using disk diffusion method. Carbapenemase, plasmidic AmpC (pAmpC), extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes were characterized by PCR. Plasmids were extracted by Kieser's extraction method and analyzed by plasmid replicons typing method (PBRT).The epidemiological relationships between E. cloacae isolates were determined by Random amplified polymorphic DNA (RAPD) analysis and Multilocus Sequence Typing (MLST).12 Enterobacteriaceae (2.2%) including two Escherichia coli, two Klebsiella pneumoniae and eight Enterobacter cloacae isolates were recovered from selection plates. The 12 strains were resistant to amoxicillin-clavulanic acid, ticarcillin, piperacillin-tazobactam, and ertapenem. All isolates were susceptible to aminoglycosides, imipenem and extended-spectrum cephalosporins. PCR and sequencing identified the blaOXA-48 gene in all isolates. qnrB1 gene was identified in all E. cloacae isolates. Plasmids analysis showed that the blaoxa-48 gene was localized on 7kb plasmid which was not typeable.RAPD analysis demonstrated the presence of the same profile pattern in the eight isolates of E. cloacae. MLST analysis showed that the E. cloacae isolates were assigned to ST 527.This study reported for the first time the presence of CPE in horses and pet birds in the world.