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Characterization of protein complexes using chemical cross-linking coupled electrospray mass spectrometry

Research paper by Timothy D. Cummins, Gopal P. Sapkota

Indexed on: 14 Jun '16Published on: 14 Jun '16Published in: Quantitative Biology - Molecular Networks



Abstract

Identification and characterization of large protein complexes is a mainstay of biochemical toolboxes. Utilization of cross-linking chemicals can facilitate the capture and identification of transient or weak interactions of a transient nature. Here we describe a detailed methodology for cell culture based proteomic approach. We describe the generation of cells stably expressing green fluorescent protein (GFP)- tagged proteins under the tetracycline-inducible promoter and subsequent proteomic analysis of GFP-interacting proteins. We include a list of proteins that were identified as interactors of GFP.