Characterisation of microsatellite loci in the rifleman (Acanthisitta chloris, Acanthisittidae, AVES) and their predicted genome locations

Research paper by Stephanie A. J. Preston, Deborah A. Dawson, Gavin J. Horsburgh, Ben J. Hatchwell

Indexed on: 12 Jan '13Published on: 12 Jan '13Published in: Conservation genetics resources


Thirty-seven unique polymorphic microsatellite loci were characterised in 24 unrelated rifleman individuals (Acanthisitta chloris) sampled from a population located at Kowhai Bush, Kaikoura, New Zealand. Thirty of these loci were newly isolated from a rifleman genomic library enriched for microsatellite motifs, and seven were identified from the testing of markers of known high-cross species utility. Sequence homology to the assembled zebra finch (Taeniopygia guttata) genome was successfully used to predict the chromosome locations for 33 of these polymorphic loci. Three of the loci were found to be Z-linked based a lack of heterozygotes in females and sequence homology. These loci will be of utility for population and parentage studies in the rifleman. A proportion of these loci are expected be useful in the related species, such as the endangered rock wren (Xenicus gilviventris).