Cationic polyacrylamides promote binding of cellulase and amylase.

Research paper by John T JT Reye, Kendra E KE Maxwell, Sujit S Banerjee

Indexed on: 21 Jun '11Published on: 21 Jun '11Published in: Journal of biotechnology


Cationic polyacrylamides (c-PAMs) increase the binding of cellulase to cellulose and of α-amylase to cornstarch. The c-PAMs increase the rate of hydrolysis of both substrates by about the same amount, which suggests that the increased binding is responsible for the enhanced rates. These results support our proposed mechanism where the c-PAM neutralizes the charge of the substrate through a "patching" mechanism, which reduces the charge repulsion between fiber and enzyme.