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Carboxylate-containing two-photon probe for simultaneous detection of extra- and intracellular pH values in colon cancer tissue.

Research paper by Ho Young HY Si, Myoung Ki MK Cho, Ji Su JS Kang, Choong-Kyun CK Noh, Sung Jae SJ Shin, Chang Su CS Lim, Hwan Myung HM Kim

Indexed on: 01 Jun '18Published on: 01 Jun '18Published in: Analytical Chemistry



Abstract

Acidified extracellular pH (pHe) is directly related to various disorders such as tumor invasion and resistance to drugs. In this study, we developed two-photon-excitable emission ratiometric probes (XBH1-3) for the in situ measurement of pHe. These probes, based on benzimidazole and polar solubilizing groups, exhibited a strong two-photon-excited fluorescence and sensitive blue-to-green emission color changes with pKa values of 5.1-5.7. XBH1, containing carboxylic acid, stained the extracellular region in neutral media; it entered the cell under acidic media, thereby allowing a precise measurement of the extra- and intracellular pH values in the acidified tissue. XBH2, containing the sulfonate peripheral unit, facilitated monitoring of the pHe value only. Ratiometric two-photon microscopy imaging re-vealed that XBH1 can directly monitor the pH values both inside and outside the cells in colon cancer tissue, as well as the morphologi-cal aspect. This could be useful for cancer analyses and drug development.