Indexed on: 21 Mar '20Published on: 21 Mar '20Published in: ChemBioChem
Cys is present in a large number of biomolecules and other synthetic molecules. Although the thiol side chain of Cys can be in a free form, in most cases it forms a disulfide bond either with a second Cys (bridge) or with another thiol, as in the case of the protecting group. Efficient reduction of these disulfide bridges is a requirement for many applications of Cys-containing molecules in the biochemical and organic chemistry arenas. Here we review reducing methods for disulfide bonds, taking into consideration the solubility of the substrates when selecting the appropriate reducing reagent. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.